"The great question that has never been answered, and which I have not yet been able to answer, despite my thirty years of research into the feminine soul, is 'What does a woman want?'"
Sigmund Freud

Selected peer review

1) Cohen-Hadar, N., Wine, Y., Nachliel, E., Huppert, D., Gutman, M., Frolow, F., Freeman, A. (2006).  Monitoring the stability of crosslinked protein crystals biotemplates: A feasibility study. Biotechnology and Bioengineering. 94, 1005-1011. Pubmed


2) Wine, Y., Cohen-Hadar, N., Freeman, A. & Frolow, F.  (2007). Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction by X-ray structure analysis. Biotechnology and Bioengineering 98, 711-718. Pubmed


3) Cohen-Hadar, N., Wine, Y., Lagziel-Simis, S., Moscovich-Dagan, H., Dror, Y., Frolow, F. & Freeman, A.  (2009). Protein Crystal-Mediated Biotemplating. Journal of Porous Media 12, 213-220. Pubmed


4) Wine, Y., Cohen-Hadar, N., Lamed, R., Freeman, A. & Frolow, F.  (2009). Modification of Protein Crystal Packing by Systematic Mutations of Surface Residues: Implications on Biotemplating and Crystal Porosity. Biotechnology and Bioengineering 104, 444-457. Pubmed


5) Cohen-Hadar, N., Lagziel-Simis, S., Wine, Y., Frolow, F. & Freeman, A.  (2011). Re-structuring protein crystals porosity for biotemplating by chemical modification of lysine residues. Biotechnology and Bioengineering 108, 1-11. Pubmed


6) Saggy, I., Wine, Y., Shefet-Carasso, L., Nahary, L., Georgiou, G. & Benhar, I.  (2012). Antibody isolation from immunized animals: comparison of phage display and antibody discovery via V gene repertoire mining. Protein Engineering Design and Selection 25, 539-549. Pubmed


7) DeKosky, B.J., Ippolito, G.C., Deschner, R.P., Lavinder, J.J., Wine, Y., Rawlings, B.M., Varadarajan, N., Giesecke, C., Dorner, T., Andrews, S.F., Wilson, P.C., Hunicke-Smith, S.P., Willson, C.G., Ellington, A.D. & Georgiou, G.  (2013). High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nature Biotechnology 31, 166-169.  Pubmed


8) Cotham, V.C., Wine, Y.,  Brodbelt, J.S.  (2013). Selective 351 nm Photodissociation of Cysteine-Containing Peptides for Discrimination of Antigen-Binding Regions of IgG Fragments in Bottom-Up Liquid Chromatography - Tandem Mass Spectrometry Workflows. Analytical Chemistry 85, 5577-5585. Pubmed


9) *Wine, Y., *Boutz, D.R., *Lavinder, J.J., Miklos, A.E., Hughes, R.A., Hoi, K.H., Jung, S.T., Horton, A.P., Murrin, E.M., Ellington, A.D., Marcotte, E.M. & Georgiou, G.  (2013). Molecular deconvolution of the monoclonal antibodies that comprise the polyclonal serum response. Proceedings of the National Academy of Sciences of the United States of America 110, 2993-2998.  * first co-author. Pubmed


10) *Lavinder, J.J., *Wine, Y., Giesecke, C.,  Ippolito, G.C., Horton, A.P, Lungu, O.I., Hon Hoi, K., DeKosky, B.J., Murrin, E.M., Wirth, M.M., Ellington, A.D., Dörner, T., Marcotte, E.M., Boutz, D.R., Georgiou, G. (2014). Identification, Dynamics, and Functionality of the Constituent Antibodies within Human Serum after Vaccination. Proceedings of the National Academy of Sciences of the United States of America. 111, 2259-2264. * first co-author. Pubmed


11) Buch, M., Wine, Y., Dror, Y., Rosenheck, S., Giordano, R., Leal, M.F.R., Popov, N.A., Freeman, A., Frolow, F. (2014). Protein Products Obtained by Site-Preferred Partial Crosslinking in Protein Crystals and “Liberated” by Redissolution. Biotechnology and Bioengineering. 111, 1296-1303. Pubmed


12) *Boutz, D.R., *Horton, A.P., *Wine, Y., Lavinder, J.J, Georgiou, G., Marcotte, E.M. (2014). Proteomic Identification of Monoclonal Antibodies from Serum. Analytical Chemistry. 86, 4758-4766.  * first co-author. Pubmed


13)  Lavinder, J.J., Hon Hoi, K., Reddy, S.T., Wine, Y., Georgiou, G. (2014). Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire. PloS one. 9, e101322. Pubmed

14) Vaisman-Mentesh, A. & Wine, Y. Monitoring Phage Biopanning by Next-Generation Sequencing. Methods in molecular biology (Clifton, N.J.) 1701, 463-473, (2018). Pubmed.

15) *Avram, O., *Vaisman-Mentesh, A., Yehezkel, D., Ashkenazy, H., Pupko, T. & Wine, Y. ASAP - A Webserver for Immunoglobulin-Sequencing Analysis Pipeline. Frontiers in Immunology 9, 1686, (2018). * first co-author. Pubmed



Invited reviews

1) Lagziel-Simis, S., Cohen-Hadar, N., Moscovich-Dagan, H., Wine, Y. and Freeman, A. (2006). Protein-mediated nanoscale biotemplating. Current opinion in biotechnology. 17, 569-573. Pubmed


2) Wine, Y., Ippolito, G.C., Horton, A.P., Georgiou, G. (2015). Serology in the 21st century: the molecular-level analysis of the serum antibody repertoire. Current Opinion in Immunology. 35: 89-97. Pubmed


Conference proceeding

1) Wine, Y., Cohen-Hadar, N.,Lagziel-Simis, S.,  Dror Y., Felix Frolow, F. and Freeman, A. (2010). Adjustment of Protein Crystal Porosity for Biotemplating: Chemical and Protein Engineering Tools. AIP conference Proceedings. 76: 198-203. Ebsco host



1) Jason Lavinder, Yariv WINE, Danny Boutz, Edward Marcotte, George Georgiou. (2014). Proteomic identification of antibodies. Patent application PCT/US2012/066454


Crystal structures

  • Crystal structure analysis of the K171A mutation of N-terminal type II cohesin 1 from the cellulosomal ScaB subunit of Acetivibrio cellulolyticus. PDB ID: 2F2L

  • Parent Structure of Hen Egg White Lysozyme grown in acidic pH 4.8. Refinement for comparison with crosslinked molecules of lysozyme. PDB ID: 2HU3

  • Structure of Hen Egg-White Lysozyme Determined from crystals grown in pH 7.5. PDB ID: 2HUB

  • Crystal Structure Analysis of Hen Egg White Lysozyme Crosslinked by Polymerized Glutaraldehyde in Acidic Environment. PDB ID: 2HTX

  • Crystal structure Analysis of Hen Egg White Lyszoyme. PDB ID: 2HU1


- Yariv Wine -